Proteomics based on high-efficiency capillary separations

Advances in Capillary Electrochromatography: Rapid and High-Efficiency Separations of PAHs

Capillary columns packed electrokinetically with 1.5-μm nonporous octadecylsilica particles are used to achieve rapid separations with high efficiencies. A mixture containing five polycyclic aromatic hydrocarbons (PAHs) as test compounds is separated in less than 5 s with 28 kV applied to a column with a packed length of 6.5 cm (10 cm total length). A sample containing 16 PAHs (classified as pr...

Chiral Separations in Capillary Electrophoresis

We carried out different research projects by using our expertise in the field of the study of equilibria in solution, the synthesis and the NMR characterisation of cyclodextrin derivatives, capillary electrophoresis techniques, and electrospray mass spectrometry for the detection and the characterisation of the involved analytes. The most of our work concerns the separation science, and partic...

A Novel Chromatography Format for High Efficiency Separations

SEPARATIONS W. De Malsche*, J. Op De Beeck, Selm De Bruyne, H. Gardeniers and Gert Desmet Vrije Universiteit Brussel, BELGIUM and Mesa+ Institute for Nanotechnology, THE NETHERLANDS ABSTRACT A non-porous C8 coated silicon pillar channel design is presented that allows the achievement of a high pressure and high efficiency operation for HPLC separations. Performing on-chip injection and detectio...

Ultra-fast DNA separations using capillary electrophoresis.

Exploration of pH-Gradient Ion-Exchange Chromatography for High-Resolution Protein Separations in Biotechnology and Proteomics

Ion-exchange chromatography (IEC) is a versatile separation technique for profiling the charge heterogeneity of biotherapeutic proteins, including monoclonal antibodies. Despite good resolving power and robustness, ionic-strength-based ion-exchange separations are product specific and time consuming to develop. Although salt gradients are more commonly applied, the utilization of pH gradients c...